A glove box is a piece of equipment that lets you perform work on a subject while keeping it in isolation. A glove box is most commonly used to protect operators working with hazardous materials, but it can also be used to protect sensitive materials from the outside atmosphere. In this project, I am going to show you how to make a DIY glove box. Since different applications require different degrees of isolation, I am going to demonstrate a variety of design features and how to implement them. You can then choose which features you want to use in your glove box.