How to make the liquid and solid growth media used to select and propagate bacteria in science labs, using only materials you can find in a day's shopping.
"Broth" is a catch-all term for any liquid medium in which we grow bacteria. The most popular for growing E.coli is "Lysogeny Broth", and I’ll show you here how to make this and other media using only off-the-shelf ingredients.
If you want to make petri dishes of agar for growing cells on a solid surface, you simply add agar flakes to the broth before you sterilise it, and then pour it before it cools too much! It couldn’t be easier.
This guide will cover:
- Finding Ingredients
- Making peptone
- Mixing broth according to a formula
- Sterilising with a pressure cooker
- Pouring plates (if using agar)
- Keeping it sterile while you work
Things you may need besides the ingredients:
- A pressure cooker, and perhaps some help using it if you’re unfamiliar with them.
- A room air-purifier with a HEPA-type filter
- An alcohol-based hand disinfectant, (or ethanol at 70% if you can get it)
- Some glassware that will survive pressure cooking. Jam jars work well.
More on this soon as I gather my notes and pictures into something manageable!Related